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Tryptone

Tryptone is a specially formulated enzymatic digest of casein by the protease trypsin. It provides a source of amino acids for the growing bacteria. Tryptone is similar to casamino acids, both being digests of casein It is equivalent to Casein Enzyme Hydrolysate.  

Application:

  It is used to supply higher growth rates when used in molecular biology media preparations such as YPD Broth and Agar. Used as a nitrogen source in culture media for fastidious and non-fastidious organisms. Also used to deferentially select bacteria based on carbohydrate intake since Tryptone is carbohydrate deficient. Tryptone is commonly used in microbiology to produce lysogeny broth (LB) for the growth of E. coli and other microorganisms. [2] It is used in the production of Sterility Testing Media such as Tryptone Soya Agar and broth. Tryptone is also a component of some germination media used in plant propagation.

Product Quality specification:

 
Description Light Brown Powder.
Solubility  Completely soluble in water with clear solution
Reaction (pH of 2% solution) 6.0 to 7.5
Total Nitrogen NLT 12.5%
Amino Nitrogen NLT 3.50%
Protein NLT 78.0%
Loss on drying NMT 5.0%
Sulphated Ash NMT 15.0%
Microbial Test
Total Bacterial Count Upto 5000 cfu/g
Pathogens (E.Coli, Salmonella & shigella) Absent
Total Yeast & Mould Count NMT 100 cfu/gm
 
Storage conditions : Store below 30°C Shelf Life:  24 months Pack sizes: 25 kg
       

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