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Brain Heart Infusion

BHI powder is highly nutritious and is applied in number of media for cultivation of highly fastidious microorganisms, such as in BHI Agar/Broth, SABHI Agar Base, etc. to grow Staphylococci, Streptococci, Haemophilus and Neisseria species and pathogenic fungi.


Used for cultivation of highly fastidious microorganisms like Streptococci,Pneumococci, Meningococci, Haemophilus, Neisseria sp. and pathogenic fungi.

A highly nutritious mixture of calf brain infusion and beef heart infusion

A number of fastidious organisms, including some bacteria, yeasts, and other fungi, grow well on BHI.

It can also be used to differentiate between enterococci and group D streptococci.

Product Quality specification:
Appearance Light yellow to brownish yellow powder, odour characteristic.
Solubility  Completely soluble in water
Clarity (1% aq solution) Clear solution
Reaction (pH of 2% solution) 5.5 - 7.0
Total Nitrogen  NLT 12.5%
Protein NLT 75%
Sulphated Ash  NMT 12%
Amino Nitrogen  NLT 3.5 %
Loss on drying NMT 5.0%
Total bacterial count NMT 5000cfu/g
Total Yeast and mould count (cfu/gm) NMT 100 cfu/g
Pathogens (E.coli, Salmonella & Shigella)  Absent
Storage conditions : Store below 30°C Shelf Life:  24 months Pack sizes: 25 kg