Potato Dextrose Broth is used for the cultivation of fungi in a laboratory setting.It is recommended for the isolation and enumeration of yeasts and moulds. Potato Dextrose Broth is not intended for use in the diagnosis of disease or other conditions in humans. Potato Dextrose Broth is a general purpose broth for yeasts and molds. The low pH of this medium inhibits bacterial growth.

Ingredients	Gms / Litrer
Potatoes, infusion	200.000
Dextrose	20.000
Final pH ( at 25°C)	5.1±0.2

  (*4.0 g of potato extract is equivalent to 200 g of infusion from potatoes.) Formula may varies as required to meet performance specifications.  

Preparation:

  Dissolve 24 g of the medium in one liter of purified water, Mix thoroughly&Autoclave at 121°C for 15 minutes.  

Product Quality specification:

 

Description	Off White Powder
Solubility	Soluble in water with cloudy solution
Reaction (pH of 2% solution)	5.0 to 6.0
Potato infusion	NLT 15.0%
Dextrose	NLT 75.0%
Loss on drying	NMT 5.0%
Microbial Test
Total bacterial count	NMT 10,000 cfu/g
Pathogens (E.Coli, Salmonella & shigella)	Absent
Total Yeast & Mould Count	NMT 100 cfu/gm

 

Storage conditions : Store below 30°C

Shelf Life:  24 Months

Pack sizes: 25 kg