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Potato Dextrose Broth

Potato Dextrose Broth is used for the cultivation of fungi in a laboratory setting.It is recommended for the isolation and enumeration of yeasts and moulds. Potato Dextrose Broth is not intended for use in the diagnosis of disease or other conditions in humans. Potato Dextrose Broth is a general purpose broth for yeasts and molds. The low pH of this medium inhibits bacterial growth.
Ingredients Gms / Litrer
Potatoes, infusion  200.000
Dextrose  20.000
Final pH ( at 25°C) 5.1±0.2
  (*4.0 g of potato extract is equivalent to 200 g of infusion from potatoes.) Formula may varies as required to meet performance specifications.  

Preparation:

  Dissolve 24 g of the medium in one liter of purified water, Mix thoroughly&Autoclave at 121°C for 15 minutes.  

Product Quality specification:

 
Description Off White Powder
Solubility Soluble in water with cloudy solution
Reaction (pH of 2% solution)  5.0 to 6.0
Potato infusion NLT 15.0%
Dextrose NLT 75.0%
Loss on drying NMT 5.0%
Microbial Test
Total bacterial count NMT 10,000 cfu/g
Pathogens (E.Coli, Salmonella & shigella) Absent
Total Yeast & Mould Count NMT 100 cfu/gm
 
Storage conditions : Store below 30°C Shelf Life:  24 Months Pack sizes: 25 kg
   

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