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Potato Dextrose Broth

Potato Dextrose Broth is used for the cultivation of fungi in a laboratory setting.It is recommended for the isolation and enumeration of yeasts and moulds.

Potato Dextrose Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Potato Dextrose Broth is a general purpose broth for yeasts and molds. The low pH of this medium inhibits bacterial growth.

Ingredients                                                  Gms / Litre Potatoes, infusion                                          200.000 Dextrose                                                     20.000 Final pH ( at 25°C)                                         5.1±0.2   (*4.0 g of potato extract is equivalent to 200 g of infusion from potatoes.)

Formula may varies as required to meet performance specifications.

Preparation:

Dissolve 24 g of the medium in one liter of purified water, Mix thoroughly&Autoclave at 121°C for 15 minutes.

 

Product Quality specification:

Appearance whitishhomogeneous powder
Clarity (1% aq solution) opalescent solution
Reaction (pH of 2% solution)  5.0–6.0
Potato infusion  NLT 16.5 %
Dextrose  NLT 83%
Loss on drying NMT 5.0%
Total bacterial count NMT 5000cfu/g
Pathogens (E.coli, Salmonella & Shigella)  Absent
 
Storage conditions : Store below 30°C Shelf Life:  24 months Pack sizes: 25 kg